We’re committed to your longevity and results, so we’re here to make NAD+ dosing as simple as possible.
Here’s how to get the most from your dose of NAD+!
Preparing Your Dose
Depending on which NAD+ protocol you selected for your medical evaluation, you will receive either a 2.5ml vial or a 5ml vial of NAD+.
Your package will contain everything you need for that month’s worth of doses, including syringes, needles, and alcohol wipes. Each of these may be packaged separately, so check the contents of your shipment thoroughly before discarding!
Before getting started, immediately refrigerate your vial. Next, be sure to review how to draw up your NAD+ solution, and remember to sterilize the top of the vial with an alcohol wipe each time.
As a subcutaneous injection, NAD+ is best administered on either your stomach or upper arm. To avoid irritation, administer the injection in a different location than the last one.
Finally, choose a dosing schedule that works for you. For example, if you’ll be injecting 3 times per week, decide whether Monday/Wednesday/Friday is a viable schedule to keep on a consistent date. Try setting alarms or calendar reminders to keep you on track!
Dosing & Titrating with NAD+
NAD+ is most commonly prescribed at a dose of 0.4ml, taken 2-3 times per week. This has been indicated as providing the best balance of improved energy and weight loss benefits for most users, with the most minimal occurrence of side effects.
However, it’s important to ease yourself into the target dose. To do this, you’ll start with a smaller dose and gradually ramp up to the target dose over time.
For your first dose, start by injecting 0.1ml. Pay careful attention to how your body responds to the initial dose to decide when it’s best to ramp up.
When you’re ready, you may titrate up to a dose of 0.25ml, up to 3 times per week. Follow the same process as above before moving up to the target dose of 0.4ml.
Maintaining Your Dose
Increasing to a dose of 0.4ml may enhance the benefits of NAD+, but this may not be true for everyone.
To determine which dose provides the best benefits for you, your prescriber may recommend that you stay on a 0.4ml dose 3 times a week for 1-2 months, then reduce to a lower maintenance dose of 0.25ml to 0.4ml 2 times per week.
As you proceed, keep in mind that you should always follow the instructions of your prescriber and never use more than 0.4ml per injection. Doses higher than 0.4ml may exacerbate side effects like anxiety, insomnia, and heart palpitations – without proportionally increasing the benefits.
As such, you shouldn’t exceed a 0.4ml dose within any 24-hour period. Keep this in mind if you miss a dose!
What Does NAD+ Stand For?
NAD+ stands for nicotinamide adenine dinucleotide, a coenzyme found in every cell that helps turn the nutrients we eat into usable energy. You may also see it written as NADH, which is a closely related form of the same molecule.
NAD+ and NADH work as a pair, cycling back and forth between an oxidized state (NAD+) and a reduced state (NADH) as cells produce energy. This cycling is part of why NAD+ is considered so important to healthy cellular function, and why supporting your levels is thought to help support energy and cellular repair as you age.
NAD+ and Brain Health
Researchers are increasingly interested in how NAD+ may relate to brain health, particularly around a protein called tau. In a healthy brain, tau helps support the structure of neurons, but in conditions involving cognitive decline, tau can become abnormal and build up inside cells.
One study looked at whether an NAD+ precursor could influence this process in people showing early signs of cognitive decline, and found that participants receiving the NAD+ precursor saw less tau buildup over the study period compared with those on a placebo, though cognitive test scores themselves didn’t show significant differences in that short window. Researchers describe this as an early but promising area of study, since supporting cellular energy metabolism, which is central to how NAD+ works, may be one piece of a broader approach to supporting brain health as we age.
Why Is Everyone Talking About NAD+?
NAD+ has become a familiar name in wellness circles, in part thanks to public figures who have talked about it, including Hailey Bieber and Joe Rogan. On an episode of The Kardashians, Bieber said she planned to keep using NAD+ as part of her routine. Interest has also grown as NAD+ has become easier to access outside of high-end clinics, with more at-home options like injections, patches, and nasal sprays now available.
Beyond the buzz, the underlying interest comes down to biology: NAD+ is a coenzyme found in every cell that supports energy production, DNA repair, and other processes tied to aging, and levels are known to decline as we get older. That’s part of why NAD+ supplementation tends to be discussed as a longevity-focused option rather than a passing trend, though as with any supplement, individual results and needs can vary.
NAD+, Glutathione, and Sun-Damaged Skin
Sun exposure does more than cause a sunburn or a tan that fades. Over time, UV rays can trigger inflammation and oxidative stress that contribute to DNA damage, collagen breakdown, and visible signs of aging like fine lines and uneven tone. Because NAD+ is involved in cellular energy production and DNA repair, and levels tend to decline with both age and prolonged sun exposure, supporting NAD+ is thought to help support skin’s ability to repair and renew itself from within.
Glutathione can play a complementary role. As one of the body’s key antioxidants, it helps neutralize free radicals generated by UV exposure, and some research suggests it may also help support a more even skin tone by influencing melanin production. Because of this, options like NAD+ Face Cream are formulated specifically with sun-damaged and aging skin in mind, meant to work alongside sunscreen and hydration rather than replace them.
NAD+ and Long COVID
Long COVID has left many people dealing with ongoing fatigue and brain fog, with few treatment options available. AgelessRx researchers studied a combination of NAD+ and Low Dose Naltrexone (LDN) to see whether it could help, using a 12-week study with participants ages 18 to 65 who tracked their symptoms with established clinical surveys.
The study found that 52% of participants using both LDN and NAD+ iontophoresis patches saw substantial improvements in energy, pain, and physical health, adding up to about a 20% improvement in overall quality of life. NAD+ is generally well tolerated, though some people may notice mild, temporary side effects like flushing, nausea, or headache, especially at higher doses. Researchers note that more studies are still needed, but the results suggest NAD+, alongside LDN, may be worth discussing with a doctor for people managing Long COVID fatigue.
NAD+ IontoPatch™: A Needle-Free Option
For people who’d rather avoid needles, the NAD+ IontoPatch™ offers another way to supplement NAD+. It’s a transdermal patch worn on the skin that uses a very small electrical charge, a method called iontophoresis, to help move NAD+ through the skin and into the bloodstream. Transdermal patches have been used for decades and are considered a well-established, generally safe drug delivery method.
Because the patch is designed to deliver NAD+ directly into the bloodstream rather than through digestion, it’s generally considered a more bioavailable option than NAD+ precursors or pills, some of which can be lost during digestion. AgelessRx offers a 4-hour IontoPatch™ kit that can simply be removed and discarded once your dosing time is complete.
NAD+ Nasal Spray and Mental Clarity
Many people first notice reduced NAD+ levels through everyday brain fog, the hazy, hard-to-focus feeling that can show up during an afternoon slump. NAD+ plays a role in helping cells communicate and produce energy, and research suggests it may help support mental clarity and overall brain health, beyond just a short-term lift.
For a needle-free way to target these effects, NAD+ Nasal Spray is designed to deliver NAD+ directly, offering a more targeted option compared with methods absorbed more broadly through the body. As with any supplement, it’s worth talking with your prescriber about which method best fits your goals.
New Research on NAD+ and Healthy Aging
A recent 26-week clinical study looked at how NAD+ supplementation affects people with Werner syndrome, a rare genetic condition that causes accelerated aging. In this placebo-controlled study, participants who took an NAD+ precursor saw notable improvements including reduced arterial stiffness, smaller chronic skin ulcers, and better kidney function compared with the placebo period, with no serious adverse effects reported.
Because Werner syndrome is often studied as a model for accelerated aging, researchers see these results as a meaningful signal for how NAD+ might support healthy aging more broadly, including possible effects on skin health and metabolic function. More research is still needed to confirm these findings in the general population, but the study adds to a growing body of evidence around NAD+ as a potential longevity-focused option.
What Are the Benefits of NAD+?
According to feedback from AgelessRx prescribers, 87% noticed an improvement in energy by their third check-in. Additionally, 79% reported an improvement to their moods, while 78% reported better memory and focus.*
But those are just the benefits you can notice from a self-evaluation! After all, NAD+ is a critical coenzyme found in every cell in our bodies, but these levels decrease with age. NAD+ injections provide a wide range of additional longevity benefits, like DNA repair, improved immunity, and reduced inflammation.
But these outcomes don’t happen overnight. The best outcomes for NAD+ injections were reported after several months of dedicated use. So if you don’t notice the outcomes you were looking for, try adjusting your dose – and most importantly, stick with it!
Of course, we’re always here to help you discover the best NAD+ dose for you. Should you ever need help with your NAD+ dosage, or if you’re curious about adding NAD+ injections to your regimen, we’re always a tap or click away!
Note: The above statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
*Data was self-reported by 193 surveyed customers on their third check-in of use between 4/7/22 & 11/27/23 by AgelessRx customers who were approved for a NAD+ injection prescription after completing an assessment with a medical provider.